Indirect Organogenesis from in vitro Derived Leaf and Internodes of Coccinia cordifolia (L.) Cogn. - An Important Medicinal Climber

An efficient in vitro regeneration protocol was developed via callus from the explants of leaf and internode Coccinia cordifolia (L.) Cogn., an important medicinal climber. Among different combinations auxins cytokines used, BAP with NAA were found highly effective for induction derived disc explants. Maximum formation rates recorded on MS 1.0 mg/l 0.1 both The best organogenesis obtained 2.0 0.5 NAA. highest numbers shoots regenerated per culture 6.5 ± 0.10 5.6 0.55 respectively discs to be better indirect organogenesis. For root induction, half strength medium supplemented IBA most effective. mean number roots shoot 6.8 average length 2.8 0.20 cm. In plantlets acclimatized successfully. This can utilized improvement this climber through biotechnological methods. Plant Tissue Cult. & Biotech. 32(2): 127-136, 2022 (December)